PrinCE with LED and Laser-Induced Fluorescence detectors
LASER Induced Fluorescence detectors are extremely powerful, typically capable of emitting light of a very well defined wavelength with an intensity of 1-40 mW. Thus the maximum possible number of molecules can be excited, resulting in the best possible limits of detection.
For fluorescent compounds, concentration limits of detection down to 10-12 mol/l have been reported. Bearing in mind that typically about 10 nl of sample is injected, this equals about 10-20 mol! LIF detection can be more sensitive than any other commercially available detector, even if the compound is not fluorescent itself. Of course a suitable label should be available.
Comparable with the LIF, LED is from 10–100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection. Low noise and interference molecules will be detected. Only labeled or native fluorescence can be measured.
The CE-cell can be placed inside the temperature controlled capillary compartment. The detector can be used with any PrinCE and PrinCE Next system.
Specifications — LIF detector
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Specifications |
LIF Details |
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Available Laser Wavelengths |
266, 325, 355, 410, 442, 473, 488, 514, 532, 594, 633, 638, 642 & 780 nm. |
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Emission Filter: |
The emission filters depend on the wavelength and generally contain highpass filters |
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LIF Capillaries |
OD 365 μm; ID from 20 to 320 μm |
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Cell Detection Volume |
The cell is a small part of the capillary and depends on the capillary ID, for example 0.4 nL with a 50 μm ID. |
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Signal Outputs |
- External Ethernet for Network communications. - External Event (detection of a relay state) function. Start/stop command port for an external event command (relay opened or closed). - Analog output: Processed: 0-1V(DC) for range 0-50 RFU. 0-100mV(DC) for range 0-5 RFU. |
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Power Requirements |
100-240 VAC, 47/63 Hz, 1.5 A |
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Dimensions and Weight |
43.0 cm / 16.9”(H) x 23.0 cm / 9.1”(W) x 34.0 cm / 13.33” (D). 12 kg / 26.4 lbs |
Specifications — LED detector
|
Specifications |
LED Details |
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Available LED Wavelengths |
450, 480, 530 nm |
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Emission Filter: |
The emission filters depend on the wavelength and generally contain highpass filters |
|
LIF Capillaries |
OD 365 μm; ID from 20 to 320 μm |
|
Cell Ddetection Volume |
The cell is a small part of the capillary and depends on the capillary ID, for example 0.4 nL with a 50 μm ID. |
|
Signal Outputs |
- External Ethernet for Network communications. - External Event (detection of a relay state) function. Start/stop command port for an external event command (relay opened or closed). - Analog output: Processed: 0-1V(DC) for range 0-50 RFU. 0-100mV(DC) for range 0-5 RFU. |
|
Power Requirements |
100-240 VAC, 47/63 Hz, 1.5 A |
|
Dimensions and Weight |
43.0 cm / 16.9”(H) x 23.0 cm / 9.1”(W) x 34.0 cm / 13.33” (D). 12 kg / 26.4 lbs |
Further information
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